• Publié par : Christian DELAMARCHE
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Ribosome Biogenesis

The ribosome is an essential ribonucleoprotein enzyme responsible for protein synthesis, and its biogenesis is a fundamental process in all living cells. Though, the details of the fine tuned assembly process still remain unknown. Investigations will focus on elucidating the cellular processes that facilitate biogenesis of the bacterial ribosomal subunits in vivo. Towards this aim, 32S and 45S particles (precursors to 50S subunits) and 21S particles (precursors to 30S subunits) will be overproduced in Escherichia coli strains carrying null mutations in either the molecular chaperones dnaK or dnaJ genes (collaborator: JH Alix, IBPC, Paris). Using single particle analysis from cryo-EM images we will describe the structures of the assembly intermediates.