• Published by: Denis CHRETIEN
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2012 November 12-16

The french Society of Microscopies organizes a series of workshops on cryo-electron microscopy of frozen-hydrated specimens.

Our team is in charge of a workshop on cryo-electron tomography of vitrified specimens, from 12-16 November 2012.

Teachers: Audrey Guesdon, Claire Heichette, Franck Bazile, Denis Chrétien

Trainees:

  • Lorène Belval, étudiante en thèse, Inra, Colmar
  • Régis Ravelle-Chapuis, Application Manager, Société JEOL
  • Solange Monier-Tschope, Inserm, Institut de Génétique et Développement de Rennes

Programme 2012:

  • Introduction to cryo-electron tomography
  • Preparationof holey carbon supports
  • Vitrification of sea urchin spermatozoïd axonems
  • Acquisition of simple-axis cryo-electron tomographic data
  • 3D reconstructions with IMOD
  • Data analysis with ImageJ
  • 3D visualisation of cryo-electron tomograms using UCSF Chimera
  • Limits and pespectives of cryo-electron tomography

The new workshop will take place in November 2013.