• Published by: Denis CHRETIEN
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Methodological developments

Cryo-electron tomography can be used to obtain 3D reconstructions of biological samples from tilt series. This method has been recently improved by the use of sensitive CCD cameras to record the cryo-electron microscope images, and as a consequence of increasing computer performances. Yet, several improvements remain to be investigated. The 3D volumes of vitrified specimens have very low signal to noise ratios, and manual segmentation may not be suited to extract the structural information present in the tomograms. One of our objectives is to design new segmentation algorithms dedicated more specifically to cryo-electron tomography. Other developments include sample preparation methods and 3D reconstructions algorithms of microtubules.
 

Selected publications:

  • Single versus dual-axis cryo-electron tomography of microtubules assembled in vitro: Limits and perspectives
    Guesdon A., Blestel S., Kervrann C. and D. Chrétien
    Journal of Structural Biology, 2013, 1:169-178.
    Weblink

  • Cryo-electron tomography of microtubules assembled in vitro from purified components
    Coquelle F., Blestel S., Heichette C., Arnal I., Kervrann C. and D. Chrétien
    Methods in Molecular Biology, 2011, 777:193-208.
    Pubmed

  • A Fourier-based method for detecting curved microtubule centers: Application to straightening of cryo-electron microscope images
    Blestel, S., Kervrann, C., and D. Chrétien
    Proceedings of the IEEE International Symposium on Biomedical Imaging: From Nano to Macro, 2009. pp. 298-301.
    Web link